Single Cell, Single Molecule, DNA and RNA Mutation Detection at Single Nucleotide Resolution
The ability to interrogate cellular heterogeneity at the single cell and single molecule levels has gained increased importance. Many bioreagents and techniques exist to address this issue separately. None has the unified approach to detect and quantify DNA and RNA changes across diverse spectrum particularly point mutations and single-nucleotide polymorphisms (SNP) while maintaining their spatial context. This challenge is even greater as different analytical platforms such fluorescence, cyto/histochemistry, flow cytometry, and microfluidics are needed to study the complexity of biological states.
Abnova has integrated padlock probe and rolling circle amplification (RCA) for in situ, single cell, single molecule, DNA and RNA mutation detection at single nucleotide resolution, and obviated the technical challenges of PCR and hybridization optimization required for efficient and target-specific analysis. Abnova provides a growing portfolio of off-the-shelf, validated mutaFISH™ probes and accessory reagents to address the unmet needs in the research and clinical settings.
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| Cell In Situ Hybridization | |
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| Circulating Tumor Cell In Situ Hybridization | |
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| Fresh Frozen Tissue In Situ Hybridization | |
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| Formalin-Fixed Paraffin-Embedded Tissue In Situ Hybridization | |
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| Flow Cytometry Mutation-Based Cell Sorting |
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| Microfluidic Cell Isolation & In Situ Hybridization |
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mutaFISH™ Probes vs Conventional DNA and RNA FISH Probes |
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Publication Reference |
- In situ mutation detection and visualization of intratumor heterogeneity for cancer research and diagnostics. Grundberg I, Kiflemariam S, Mignardi M, et al. Oncotarget. 2013;4(12):2407-18.
- In situ detection and genotyping of individual mRNA molecules. Larsson C, Grundberg I, Söderberg O, Nilsson M. Nat Methods. 2010;7(5):395-7.
- Lock and roll: single-molecule genotyping in situ using padlock probes and rolling-circle amplification. Nilsson M. Histochem Cell Biol. 2006;126(2):159-64.
- In situ genotyping individual DNA molecules by target-primed rolling-circle amplification of padlock probes. Larsson C, Koch J, Nygren A, et al. Nat Methods. 2004;1(3):227-32.
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For any inquiry, please contact : OEM@abnova.com